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The controlled application of CO2 and fertilizers through slow-release particles has been an innovative and promising fertilization strategy for crops as it allows the controlled release of CO2 in the leaf mesophyll via stomata or epidermis. The effect of the application of a CO2-enriched product in three differential concentrations based on the optimal dose (TTO1-50%, TTO2-100% and TTO3-200%) on dry biomass content, gas exchange, actual Photosystem II Efficiency and mineral nutrition was evaluated in coffee plants. The plants were sprayed with a frequency of 15 days from the emergence of the first pair of true leaves (30 DDS- BBHC12) to six months (180 DDS-BBCH19) in a standard nursery. The total biomass of 15 plants was quantified for each treatment and control at 180 DDS. There was no statistical evidence of significant differences in total biomass content (gl=3, 56; F= 0.669; p=0.575, ?=0.05). Gas exchange and PSII were mainly influenced by phenology, rather than by the application of treatments (stomatal conductance- gs: p=2.85 x 10-5, net assimilation rate- A: p=9.01 x 10-16, transpiration- E: p=8.63 x 10-4 and PSII: p=1.93 x 10-19). The macro and micronutrient foliar content of coffee was affected after the application of carbonated CO2. The application of the carbonated product did not increase the total dry biomass content of coffee plants during their establishment in standard nursery.