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Storing coffee seeds under ideal conditions of temperature and relative humidity maintains the physiological quality for their propagation over extended periods of time. The objective of this research was to evaluate the effect of three environments with contrasting temperature and relative humidity on the viability of the seed, during 14 storage months, by means of: the topographic test with tetrazolium chloride; germination by the emergence of the radicle in plastic boxes and by counting the seedlings in a sand germinator; and vigor by morphometric measurements in seedlings of Coffea arabica L . var Castillo®. For the viability of the seed, the categories of absolute staining (A) and nostaining (E) were different regardless of the environments and the storage effect. The emergence of the radicle and germination indicated that the environmental condition 10 ± 2 ° C; 65 ± 7% RH favors greater proliferation of seeds with emerging radicles and seedlings (> 75%) in the 400 and 320 days, respectively. Regarding the measurement of the morphometric variables, only the length of the hypocotyl and the taproot showed a descriptive trend by treatment and storage month. The environmental condition 10 ± 2 ° C; 65 ± 7% RH preserved the physiological quality of the seeds for propagation for four months.